FastQCFastQC Report
Wed 20 Apr 2022
EGAF00005431850

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005431850
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences128273954
Sequences flagged as poor quality0
Sequence length50-75
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCGTCG110200.021.88863610
GCGTCGT89600.021.25813711
GATCGGA156900.020.6392821
CGTCGTG106350.018.09365812
ATCGGAA196550.016.505862
TCGTGTA125450.014.40030314
GTCGTGT140250.013.95358813
CGTGTAG183950.013.21950115
CAGTACG122450.012.90402435
AGAGCGT194750.012.26767358
AAGAGCG246050.012.2368867
GAGCGTC226200.010.5767239
GATCGGG124000.010.4302961
TCGGAAG322250.010.2190573
AGCGGCG150850.010.02373310
AGTACGT130750.09.9584336
CGGAAGA355900.09.491824
ACTACCG74250.08.96219121
ATCGGGA141200.08.8333992
GCGGCGT96800.08.62134511
Produced by FastQC (version 0.11.5)