FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005431851

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005431851
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences23811452
Sequences flagged as poor quality0
Sequence length76
%GC48

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTACGT44450.032.59888535
TACGTAA47500.030.5081337
TCGTATG45800.030.4910646
CGTATGC47800.029.21522147
CGTAATC49450.028.80776839
ACCGTAC52500.028.53475633
ACGTAAT50600.028.22180238
GTACGTA52350.028.21345336
CCGTACG52800.028.10734434
TATGCCG50450.027.96170649
CTCGTAT50550.027.62586845
TCTCGTA58950.023.8672244
AATCTCG61200.023.39223142
CACCGTA66850.022.56680932
ATCTCGT65550.021.51945143
ACACGTC69850.021.440914
GATCGGA61300.020.8437962
AGATCGG64100.019.9446581
ACGTCTG76550.019.88402216
GTCACCG81550.018.66979230
Produced by FastQC (version 0.11.5)