FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005431852

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005431852
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences23811452
Sequences flagged as poor quality0
Sequence length76
%GC48

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATCA38550.033.9847250
GCCGTAT40950.032.76035748
GCGTCGT48100.028.82106212
CGCCGTA47550.027.99317447
TCGCCGT48700.026.54358546
CCGTATC52300.025.78596549
AGCGTCG54600.025.3265611
CGTCGTG55900.024.9269213
GATCGGA59200.024.2904952
GTCGCCG58200.023.17055945
TGGTCGC57850.022.64963743
AGATCGG64300.021.9733451
AGAGCGT64850.021.9559299
TCGTGTA66500.020.8282315
ATCGGAA71100.020.1008593
GTCGTGT69150.019.64423214
CGTGTAG70650.019.29803316
GTAGATC73400.019.18559332
GAGCGTC74600.018.90744410
GATCTCG76800.018.88111135
Produced by FastQC (version 0.11.5)