FastQCFastQC Report
Wed 20 Apr 2022
EGAF00005431853

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005431853
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences126730209
Sequences flagged as poor quality0
Sequence length50-75
%GC46

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCGTAT148100.031.7047544
GATCGGA173700.028.4678121
GTCACGA186200.025.6528429
ATCGGAA208150.024.921782
TCTCGTA191400.024.4697943
ACGAGAT211950.022.05644432
CGAGATT216200.021.84393333
CGTCTGA228850.021.24523516
ACACGTC230700.021.21783813
ATCTCGT220400.021.00585242
AGTCACG230550.021.00427828
TCACGAG241400.019.68761430
ACGTCTG257350.019.15952115
CACGAGA253050.018.6040131
TCGGAAG307250.017.139443
CACGTCT293400.017.03963914
CATCTCG291500.016.13863441
GCACACG328150.015.35409211
CGGAAGA343850.015.3148664
CACACGT328800.015.23276512
Produced by FastQC (version 0.11.5)