FastQCFastQC Report
Wed 20 Apr 2022
EGAF00005431854

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005431854
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences126730209
Sequences flagged as poor quality0
Sequence length50-75
%GC46

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN1643110.12965416951218No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGTCGT73100.015.301887512
CGTCGTG92100.012.53929513
AGCGTCG92300.012.18237911
TCGTGTA103250.011.21325815
GATCGGA109150.010.6657892
ATATGGG414800.09.94057644
GTCGTGT118900.09.81585714
AGATCGG118850.09.72318651
AATACCC317700.09.57887644
AATAGGG337250.09.47342944
TATAGGG249650.09.41891844
CGTGTAG129800.09.22349316
TATACCC252500.08.96413344
AAATGGG885800.08.67279144
ATAACCC264500.08.56891344
TACACCC279100.08.55550744
ATCGGAA146150.08.37931253
ATTACCC274700.08.37223444
ATTAGGG311200.08.23849744
AATTGGG445650.08.00652844
Produced by FastQC (version 0.11.5)