FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005431857

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005431857
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences45429893
Sequences flagged as poor quality0
Sequence length50
%GC50

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATGC72900.026.37411144
TCGAACG52250.021.5124740
TCGTATG91300.021.05863843
CTCGTAT93250.020.02810542
GATCGGA102500.018.653611
ACGGTAA62500.018.4083844
TTGAACG67950.017.8035437
AACGGTA65050.017.51749643
CGAACGG66300.017.15270841
ACACGTC112900.017.00754213
GTCGAAC67600.016.88805839
ATCTCGT111950.016.44587740
ACGTCTG118800.016.1999215
GAACGCT74150.016.047349
GAACGGT71250.015.9928542
AGTCGAA71300.015.9811638
TCTCGTA115000.015.91406641
ATCGGAA125500.015.4455572
AGATCGG69750.014.9607251
CACGTCT131900.014.80776214
Produced by FastQC (version 0.11.5)