FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005431858

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005431858
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences70123442
Sequences flagged as poor quality0
Sequence length75
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCGGAAGAGCACACGTCTGAACTCCAGTCACTTACAGGAATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAA9170491.307763814560044TruSeq Adapter, Index 4 (97% over 36bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTTACAGGAATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAA9122481.3009173166371382TruSeq Adapter, Index 4 (97% over 37bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCGGA1361650.049.7066761
TCGTATG2005850.033.93427744
TATGCCG2005700.033.89465747
CGTATGC2033950.033.45363245
CTCGTAT2031900.033.4517343
GTATGCC2059100.033.04403746
AATCTCG2012250.032.81899340
CACTTAC2079950.032.7321630
TCACTTA2098350.032.421129
ACTTACA2111350.032.25026731
TCTCGTA2062950.032.10872742
CGTCTGA2110400.032.0939715
ACGTCTG2116450.032.01391614
ATCTCGT2074250.031.94451741
GCCGTCT2126550.031.8922550
ACACGTC2127250.031.83246412
ATGCCGT2153850.031.58892848
CACGTCT2170650.031.26540413
CTTACAG2183450.031.25166732
CCGTCTT2167000.031.25052351
Produced by FastQC (version 0.11.5)