FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005431880

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005431880
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences70657135
Sequences flagged as poor quality0
Sequence length75
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCGGAAGAGCACACGTCTGAACTCCAGTCACATGTAAGTATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAA16980732.403257646945351TruSeq Adapter, Index 15 (97% over 40bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATGTAAGTATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAA12757581.8055614624057428TruSeq Adapter, Index 15 (97% over 41bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCGGA1686000.054.0961341
TATGCCG3157550.039.1778147
TCGTATG3169700.039.0519544
CGTATGC3186700.038.83645245
CTCGTAT3196650.038.6665143
TAAGTAT3157700.038.395936
TATCTCG3157500.038.3410740
GTATGCC3229250.038.33036446
GTAAGTA3244750.038.1653835
AAGTATC3195550.037.9095337
TCTCGTA3199100.037.8551742
ATGCCGT3266150.037.8466248
CGTCTGA3273100.037.81412515
GCCGTCT3266550.037.75469650
ATCTCGT3212650.037.71940241
ACGTCTG3282350.037.7117114
ACACGTC3316050.037.36293812
ATGTAAG3318950.037.35558333
ACATGTA3323300.037.2617431
GTATCTC3262450.037.14574439