FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005431881

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005431881
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences70657135
Sequences flagged as poor quality0
Sequence length75
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTCATAGAGTGTGTAGATCTCGGTGGTCGCCGTATCATTAAAAAAA17534582.4816432197540985Illumina Single End PCR Primer 1 (96% over 32bp)
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTCATAGAGTGTGTAGATCTCGGTGGTCGCCGTATCATTAAAAAA13092121.8529084146986712Illumina Single End PCR Primer 1 (96% over 32bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCGGA1711400.053.4346471
CGCCGTA3167000.038.95861457
GCCGTAT3172100.038.90350758
CGTATCA3181550.038.7891560
CCGTATC3210050.038.44978759
GTAGATC3220050.038.40989342
TCTCGGT3222200.038.40035647
ATAGAGT3233850.038.28399733
GTCATAG3237100.038.24766530
CGTGTAG3242700.038.17958514
TAGATCT3240150.038.1705643
GTATCAT3247600.038.0246661
TGTCATA3255900.037.99812729
TCGCCGT3248200.037.9867556
GTAGGGA3262850.037.91744617
TAGAGTG3267950.037.89190334
GTCGCCG3264500.037.86683355
GGTCGCC3266200.037.85768554
GCGTCGT3277300.037.8290410
TCGTGTA3277200.037.7734213