Basic Statistics
Measure | Value |
---|---|
Filename | EGAF00005431881 |
File type | Conventional base calls |
Encoding | Sanger / Illumina 1.9 |
Total Sequences | 70657135 |
Sequences flagged as poor quality | 0 |
Sequence length | 75 |
%GC | 51 |
Per base sequence quality
Per tile sequence quality
Per sequence quality scores
Per base sequence content
Per sequence GC content
Per base N content
Sequence Length Distribution
Sequence Duplication Levels
Overrepresented sequences
Sequence | Count | Percentage | Possible Source |
---|---|---|---|
ATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTCATAGAGTGTGTAGATCTCGGTGGTCGCCGTATCATTAAAAAAA | 1753458 | 2.4816432197540985 | Illumina Single End PCR Primer 1 (96% over 32bp) |
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTCATAGAGTGTGTAGATCTCGGTGGTCGCCGTATCATTAAAAAA | 1309212 | 1.8529084146986712 | Illumina Single End PCR Primer 1 (96% over 32bp) |
Adapter Content
Kmer Content
Sequence | Count | PValue | Obs/Exp Max | Max Obs/Exp Position |
---|---|---|---|---|
GATCGGA | 171140 | 0.0 | 53.434647 | 1 |
CGCCGTA | 316700 | 0.0 | 38.958614 | 57 |
GCCGTAT | 317210 | 0.0 | 38.903507 | 58 |
CGTATCA | 318155 | 0.0 | 38.78915 | 60 |
CCGTATC | 321005 | 0.0 | 38.449787 | 59 |
GTAGATC | 322005 | 0.0 | 38.409893 | 42 |
TCTCGGT | 322220 | 0.0 | 38.400356 | 47 |
ATAGAGT | 323385 | 0.0 | 38.283997 | 33 |
GTCATAG | 323710 | 0.0 | 38.247665 | 30 |
CGTGTAG | 324270 | 0.0 | 38.179585 | 14 |
TAGATCT | 324015 | 0.0 | 38.17056 | 43 |
GTATCAT | 324760 | 0.0 | 38.02466 | 61 |
TGTCATA | 325590 | 0.0 | 37.998127 | 29 |
TCGCCGT | 324820 | 0.0 | 37.98675 | 56 |
GTAGGGA | 326285 | 0.0 | 37.917446 | 17 |
TAGAGTG | 326795 | 0.0 | 37.891903 | 34 |
GTCGCCG | 326450 | 0.0 | 37.866833 | 55 |
GGTCGCC | 326620 | 0.0 | 37.857685 | 54 |
GCGTCGT | 327730 | 0.0 | 37.82904 | 10 |
TCGTGTA | 327720 | 0.0 | 37.77342 | 13 |