FastQCFastQC Report
Wed 20 Apr 2022
EGAF00005431882

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005431882
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences219125176
Sequences flagged as poor quality0
Sequence length76
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGTATG400900.019.961646
TATGCCG396700.019.46085249
CGTATGC423250.018.49555847
CTCGTAT441650.018.37059245
GATCGGA529900.016.9718342
AGATCGG554200.016.1802431
AATCTCG581050.015.93812942
TCTCGTA526150.015.7869544
ACACGTC638250.015.5612414
ATCGGAA619400.014.5315863
ACGTCTG695700.014.431375516
ATCTCGT619500.013.79893543
CACACGT794100.012.7530813
CACGTCT792800.012.62864915
CGTCTGA840850.012.18496817
ATGCCGT650600.011.58196950
GCACACG931900.011.14809412
TCGGAAG854300.011.142134
GTATGCC765050.010.87529848
CCGGGAT464900.010.44021
Produced by FastQC (version 0.11.5)