FastQCFastQC Report
Wed 20 Apr 2022
EGAF00005431888

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005431888
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences134106011
Sequences flagged as poor quality0
Sequence length50-75
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGATCGG2784950.056.3289381
CTCGTAT1618000.049.854844
CGTCTGA4252950.037.55192617
GATCGGA4182350.037.483222
ATCGGAA4179400.037.0583883
TCGTATC4079050.036.69406539
ATTCGTA4107500.036.6467237
GTCACGA4200850.036.6453130
TCGGAAG4223400.036.6133354
ACACGTC4253250.036.57444414
TATCTCG4098950.036.51715542
GAATTCG4175650.036.4560835
CGTATCT4110600.036.4051840
ACGAATT4189850.036.3920633
AATTCGT4142400.036.3862636
CGAATTC4189900.036.38174434
CACGAAT4196350.036.34657332
CGGAAGA4252650.036.331695
TTCGTAT4167450.036.32766338
AGTCACG4251100.036.26684629
Produced by FastQC (version 0.11.5)