FastQCFastQC Report
Wed 20 Apr 2022
EGAF00005431890

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005431890
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences115006077
Sequences flagged as poor quality0
Sequence length50-75
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCGTAT116100.026.0957544
AGATCGG148500.026.0737461
TCGTATC165150.022.11019939
GATCGGA177900.021.5040382
TATCTCG176300.020.96827342
GTCACGA184200.020.64112530
ATTCGTA179100.020.50644137
CGTATCT192750.019.10061540
TCTCGTA201800.018.58738544
ACGAATT202400.018.57775333
AATTCGT201750.018.50316436
GAATTCG205700.018.32393535
CACGAAT210900.017.91451832
ATCGGAA212250.017.8432563
CGAATTC211150.017.82237434
ACACGTC220550.017.59364714
TTCGTAT213700.017.58144838
CGTCTGA223800.017.21485317
AGTCACG226350.016.98161729
ATCTCGT224850.016.89697843
Produced by FastQC (version 0.11.5)