FastQCFastQC Report
Wed 20 Apr 2022
EGAF00005431891

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005431891
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences115006077
Sequences flagged as poor quality0
Sequence length50-75
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGTCGT99300.035.18599312
AGCGTCG113750.031.35146911
CGTCGTG116450.030.367213
TCGTGTA137050.025.4755315
GATCGGA144550.025.4074422
AGATCGG147600.025.0487231
GTCGTGT143300.024.42518214
CGTGTAG155000.022.83706716
ATCGGAA175250.021.096113
AGAGCGT184900.019.594499
GAGCGTC201950.018.08683210
AAGAGCG217750.016.953648
TCGGAAG273100.013.7036454
GATAGGG277700.013.27784936
CGGAAGA294500.012.9854885
GGATAGG289750.012.7884935
GTAGGAT309350.011.65582332
TAGGATA335750.010.96453433
GTAGGGA366350.010.53776119
AGGATAG356900.010.45015934
Produced by FastQC (version 0.11.5)