FastQCFastQC Report
Wed 20 Apr 2022
EGAF00005431902

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005431902
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences76636082
Sequences flagged as poor quality0
Sequence length75
%GC48

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGTATG166750.026.06602944
TATGCCG167050.025.85442447
CTCGTAT166650.025.4192843
CGTATGC179900.024.46750645
AGATCGG181450.023.5474131
TCTCGTA195950.021.91729242
GATCGGA199300.021.5370332
AATCTCG205950.020.86940240
ACACGTC213650.020.63386514
ATCTCGT224000.019.28022441
ACGTCTG238250.018.57669616
ATGCCGT249650.017.71468548
CACACGT267300.016.87961613
ATCGGAA255150.016.809253
CACGTCT266800.016.66580215
GCCGTCT276350.016.0030550
GCACACG291550.015.71226512
CCGTCTT290400.015.22879651
CGTATCG48450.015.1690583
GTATGCC302550.014.95938246
Produced by FastQC (version 0.11.5)