FastQCFastQC Report
Wed 20 Apr 2022
EGAF00005431903

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005431903
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences76636082
Sequences flagged as poor quality0
Sequence length75
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATCA135150.031.98647950
GCCGTAT141050.030.96554448
CGCCGTA157850.027.6483847
TCGCCGT164450.026.3511946
GCGTCGT166650.026.18756312
GATCGGA184450.024.9652182
CCGTATC178800.024.66009749
TGGTCGC177200.024.47454643
AGCGTCG182700.023.86830311
AGATCGG182100.023.8460031
GTCGCCG187250.023.54797245
CGTCGTG195450.022.54081513
AGAGCGT199750.022.0877469
TCGTGTA228000.020.44310415
GTCGTGT226950.020.40060214
GAGCGTC223350.019.7228410
CGTGTAG241300.019.63170216
ATCGGAA239700.019.3546983
GTAGATC241200.018.5159232
GTGGTCG237750.018.45908742
Produced by FastQC (version 0.11.5)