FastQCFastQC Report
Wed 20 Apr 2022
EGAF00005431906

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005431906
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences181100639
Sequences flagged as poor quality0
Sequence length76
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATCG166600.015.2246363
TATATCG203300.015.18611635
ATATCGC216950.014.31106236
GTATCGA191300.013.9034084
CGATAAC207300.012.6333711
CTCGTAT225350.012.62949944
GCGCTAA160600.012.5427142
TATGCCG230850.012.43698448
TCGTATG241250.011.86995545
TATCGCT268250.011.82132437
CCCGTAT273750.011.3049881
ATACGAT169500.011.2990458
CGCTAAG184550.011.14311343
CATACGA175700.011.09886257
GTCGTTA171900.010.93890220
CGATACT180500.010.51383361
CCGTATC273550.010.4089282
TCTCGTA279800.010.32226843
CGGAAAT403000.010.2944021
CGCGTAT33150.010.2074541
Produced by FastQC (version 0.11.5)