FastQCFastQC Report
Wed 20 Apr 2022
EGAF00005431907

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005431907
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences181100639
Sequences flagged as poor quality0
Sequence length76
%GC48

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATCG162600.015.63054853
GTATCGA185950.014.835414
TATATCG207450.014.28045635
ATATCGC215650.013.90059536
CGATAAC208400.013.5407511
CCCGTAT276350.011.8076071
GCCGTAT178250.011.74022148
GCGCTAA173800.011.60495642
CGTATCA184500.011.5112550
ATACGAT165350.011.21519358
TATCGCT271500.011.01519237
GTCGTTA173350.010.98534720
CATACGA176200.010.72325457
GACGCTA232850.010.6445324
CGACCGT110350.010.5017361
CGATACT177350.010.43628961
CGCTAAG193800.010.40644543
CGGAAAT407750.010.1233741
ACGCTAC250750.010.0103135
CGAAAAT411450.09.589861
Produced by FastQC (version 0.11.5)