FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005431908

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005431908
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences32524884
Sequences flagged as poor quality0
Sequence length50
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACCTAC44950.017.07986535
TTACCTA50300.015.21925134
CCTTACC53300.014.73407932
CTTACCT57200.013.72948333
CCGGTAT40850.012.740651
TACGGTA47050.012.43760344
AGTTACG64250.012.2229046
ACCTACA65800.012.06926136
GTCCGTT29550.012.015451
CCCGGTT48050.011.7494681
GTTACGG66900.011.7387577
GTCGGTT31600.011.6546981
TGCCGAC67250.011.61238522
TTACGGA71100.011.385668
CGAAACG10600.010.9992243
AACCGCG18450.010.85075144
TTACGGT55800.010.84242843
CCTTACG54900.010.77981641
TCGGATT37350.010.7783562
GTCCGAT27100.010.6604031
Produced by FastQC (version 0.11.5)