FastQCFastQC Report
Wed 20 Apr 2022
EGAF00005431920

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005431920
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences200604864
Sequences flagged as poor quality0
Sequence length76
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGTATG263550.021.03333746
CGTATGC283400.019.46177747
TATGCCG285400.019.37432349
CTCGTAT295000.018.79537845
CGTATCG170300.017.1082573
GTATCGA191750.016.4070664
TCTCGTA372350.015.1735744
TATCTCG370100.015.0383242
CCCGTAT241600.014.4588741
ACACGTC420950.013.96940914
GATCGGA379350.013.9073882
AGTCACG420300.013.87834329
GTCACGT430000.013.5488930
ATCTCGT424200.013.21961943
AGATCGG400450.013.1510391
ACGTCTG468950.012.68380916
CGACCGT118000.012.0387341
CCGTATC274000.011.6916872
TCACGTG523650.011.4554431
CACGTCT536200.011.11252615
Produced by FastQC (version 0.11.5)