FastQCFastQC Report
Wed 20 Apr 2022
EGAF00005431921

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005431921
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences200604864
Sequences flagged as poor quality0
Sequence length76
%GC48

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCCGTAT222500.024.17475148
CGTATCA243700.022.24012650
GCGTCGT280550.019.43318412
CGCCGTA296300.018.48954247
CGTATCG175700.017.5707233
GTATCGA192500.017.0376764
TCGCCGT322800.016.58417546
TGGTCGC325400.016.30202543
GATCGGA343100.016.1701052
AGCGTCG350150.015.68003911
CGTCGTG366100.015.121840513
CCCGTAT245250.014.7444891
CCGTATC379700.014.57931149
GTCGCCG401900.013.74742145
AGAGCGT414100.013.5531759
AGATCGG404500.013.3878311
GACGCTA168600.012.6225614
CGACCGT127700.012.6097181
GTAGATC468800.012.19109232
TCGTGTA489500.011.75268815
Produced by FastQC (version 0.11.5)