FastQCFastQC Report
Wed 20 Apr 2022
EGAF00005431923

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005431923
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences141616351
Sequences flagged as poor quality0
Sequence length76
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATATCG115500.012.00625935
CGTATCG78900.011.4468513
CGATAAC120950.011.259631
ATATCGC127550.011.09223936
CTCGGAT306550.010.5409761
CGACGAT111550.010.4823191
CTCGTAT111650.010.3475061
CTCAGAT848150.010.071581
CTCGGTT240700.010.0067441
CCCGTAT145250.09.9784861
GTCGTTA104150.09.78087320
CTCGGGT289800.09.3985671
CGGAAAT240200.09.3425511
CTCAAAT823150.09.3312131
CGACAAT138050.09.2055691
CTGGAAT1069900.09.2046441
TATCGCT155550.09.16296337
CTCGTTT247250.09.1469581
CGAAGAT386500.09.1304351
CTTGGAT845950.09.1128251
Produced by FastQC (version 0.11.5)