FastQCFastQC Report
Wed 20 Apr 2022
EGAF00005431926

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005431926
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences125951550
Sequences flagged as poor quality0
Sequence length75
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACACAGTGATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAA3025140.24018283220809908TruSeq Adapter, Index 5 (100% over 63bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACACAGTGATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAA2952440.23441077144346378TruSeq Adapter, Index 5 (100% over 63bp)
CTCAGATTGAACGCTGGCGGCAGGCCTAACACATGCAAGTCGAACGGTAACAGGAAGAAGCTTGCTTCTTTGCTG1596460.12675191373190722No Hit
CTTCGCAGTAACACCAAGTACAGGAATATTAACCTGTTTCCCATCGACTACGCCTTTCGGCCTCGCCTTAGGGGT1308190.10386454156379972No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGATCGG660050.039.973661
TTGAACG506200.038.5125057
AACGCTG555450.034.6984810
ATTGAAC646650.031.4492266
TCGAACG615250.030.96761740
CTCGTAT1027300.030.20086942
ACACGTC1055000.029.97257613
TATGCCG1054400.029.65633246
ACGGTAA645000.029.60170744
GTCGAAC649800.029.33878339
TCGTATG1096100.028.98783743
GAACGCT671800.028.9355149
CAGATTG710150.028.7972243
ACGTCTG1103200.028.65034515
CGGTAAC668500.028.49402845
ACGCTGG677900.028.40557711
GATCGGA1135900.028.2662051
ATCTCGT1065000.028.2035240
CGTATGC1130050.028.10167344
CACGTCT1127900.028.08145314
Produced by FastQC (version 0.11.5)