FastQCFastQC Report
Wed 20 Apr 2022
EGAF00005431927

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005431927
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences125951550
Sequences flagged as poor quality0
Sequence length75
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCAGATTGAACGCTGGCGGCAGGCCTAACACATGCAAGTCGAACGGTAACAGGAAGAAGCTTGCTTCTTTGCTG1423940.11305458329016198No Hit
CTTCGCAGTAACACCAAGTACAGGAATATTAACCTGTTTCCCATCGACTACGCCTTTCGGCCTCGCCTTAGGGGT1282330.10181137111849754No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGATCGG621050.041.745071
TTGAACG481000.037.061357
AACGCTG525000.033.52756510
CGTATCA904550.033.1194449
GCCGTAT921300.032.41708447
TCGCCGT927900.032.2649245
GTCGCCG961950.031.3450944
TGGTCGC986000.030.61084742
TCGAACG585000.029.6092140
ATTGAAC627500.029.5137356
CCGTATC1015050.029.45329548
GTAGATC1055950.029.23171431
GATCTCG1062500.028.94995134
AGAGCGT1040950.028.9189538
TCGGTGG1089750.028.8857538
GATCGGA1063550.028.5323811
TCTCGGT1097300.028.39991236
ACGGTAA617950.028.07623744
GTCGAAC618500.028.05534239
GAACGCT635300.027.9188399
Produced by FastQC (version 0.11.5)