FastQCFastQC Report
Wed 20 Apr 2022
EGAF00005431930

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005431930
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences144445699
Sequences flagged as poor quality0
Sequence length76
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATCG125400.014.691113
GTATCGA139250.013.9887964
CGACCGT83200.012.8779021
CCCGTAT185000.012.6881241
TCGTATG141850.011.72631244
CGCGGGT205000.011.2955251
CGTATGC152550.011.1109145
TCTATCG112950.010.65553162
TATATCG118900.010.5623635
CCGTATC209450.010.4474472
TATGCCG160400.010.41777247
CTATCGC120400.010.19900963
GTCGTTA148000.010.15591920
CTCGTAT165000.010.14487843
CGATAAC161550.09.9265731
TATCGCC126000.09.69032964
GTACGAT181850.09.65213325
CAACGTA221850.09.6060844
CTCGGAT340150.09.5533431
CGGGGTT382950.09.4427861
Produced by FastQC (version 0.11.5)