FastQCFastQC Report
Wed 20 Apr 2022
EGAF00005431931

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005431931
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences144445699
Sequences flagged as poor quality0
Sequence length76
%GC48

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGACCGT85900.014.9165871
CGTATCG129500.014.7323233
GTATCGA136750.013.823574
CCCGTAT194600.012.88106251
CGTATCA146400.011.68713750
GCCGTAT146250.011.31812148
TATATCG125950.011.03771635
CGCGGGT216850.010.6391641
CGATAAC164250.010.5080721
GCGTCGT165050.010.33972812
GTCGTTA149400.010.28625420
GACGCTA132650.010.0283664
CTCGGGT276750.09.8164761
ATATCGC145350.09.75771136
CGCCGTA171550.09.692547
CTCGGAT340550.09.5091651
TATCGAA232350.09.4014635
CTCGGTT209400.09.1284591
GTACGAT171050.09.09053425
ATCGAAG313150.08.9989336
Produced by FastQC (version 0.11.5)