FastQCFastQC Report
Wed 20 Apr 2022
EGAF00005431933

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005431933
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences76787240
Sequences flagged as poor quality0
Sequence length75
%GC48

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATCA70100.019.04432150
GCCGTAT79250.017.01942848
GCGTCGT90550.015.55188612
CGTATCG73500.014.9267113
GTATCGA79850.014.6473864
GATCGGA104750.013.93121052
AGCGTCG111950.012.54802711
CCCGTAT104950.012.4910721
TCGCCGT113000.012.24127346
TGGTCGC118100.011.59582243
CGCCGTA118050.011.51306447
GCGCTAA54950.011.11124942
AGAGCGT131550.010.9906639
CGACCGT46650.010.944821
CGTCGTG135800.010.80187313
GTCGCCG135100.010.41764945
CGCGAGT45050.010.1848691
CGATAAC80150.010.0718881
GTCGTTA68550.010.0671520
ATCGGAA142650.09.91599753
Produced by FastQC (version 0.11.5)