FastQCFastQC Report
Wed 20 Apr 2022
EGAF00005431934

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005431934
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences114531257
Sequences flagged as poor quality0
Sequence length76
%GC48

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCGA143700.019.3045794
CGTATCG146750.017.6046563
TCTATCG115650.017.38972362
TATATCG137400.016.31112135
CCCGTAT194900.015.3655541
TATCGCC137650.014.76222264
CGCCTAT151150.014.66592167
GTCGTTA149150.014.34173420
CGACCGT74300.014.2592491
GACGCTA118150.013.890374
CTATCGC151350.013.84053863
ATATCGC165200.013.6929136
CGATAAC167200.013.5601031
CCGTATC210600.013.4450992
ACGCTAC128650.013.356595
ATCGAAG287000.011.9379486
TCGTTAC186800.011.7916521
TATCGAA254800.011.7367085
ATAACGT179050.011.5312423
GCGCTAA97250.011.51836242
Produced by FastQC (version 0.11.5)