FastQCFastQC Report
Wed 20 Apr 2022
EGAF00005431935

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005431935
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences114531257
Sequences flagged as poor quality0
Sequence length76
%GC48

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCGA139500.019.2003944
TCTATCG118750.017.94353962
CGTATCG149550.017.6987483
TATATCG141550.016.05315435
CGACCGT78800.015.7750851
CCCGTAT194500.015.4107491
TATCGCC142600.015.16156764
CGCCTAT159700.014.74303767
CTATCGC151850.014.53824763
GACGCTA122350.013.4784474
ATATCGC168650.013.4329836
CCGTATC227050.012.9996432
GTCGTTA146350.012.96342520
CGATAAC166800.012.6797451
ACGCTAC137350.012.210365
ATCGCGC211350.011.95120767
GCGCTAA99650.011.63021842
CGCGGGT163950.011.5546141
ATCGAAG290600.011.4216436
TATTGCG184050.011.41438742
Produced by FastQC (version 0.11.5)