FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005431937

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005431937
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences66337066
Sequences flagged as poor quality0
Sequence length75
%GC47

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATCG81250.020.5839083
GTATCGA92950.018.4008054
CGTATCA80250.017.27722550
GCCGTAT83700.016.11234548
CCCGTAT109850.015.9138141
GCGTCGT92100.014.76075512
CGCCGTA104850.013.12495447
TATCGAA149550.012.5442675
AGCGTCG111250.012.37413611
ATCGCGC126050.012.37381867
TCGCCGT110400.012.31141446
GATCGGA117100.011.9847842
ATCGAAG179600.011.9656846
TGGTCGC111850.011.93718343
GTCGCCG114100.011.822144545
AATCGGG87700.011.3246745
CGTGTTA137550.011.18491659
CATCGCG139250.011.174767566
CGTCGTG124800.011.14304413
CAATCGG75600.010.8557454
Produced by FastQC (version 0.11.5)