FastQCFastQC Report
Wed 20 Apr 2022
EGAF00005431938

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005431938
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences95596809
Sequences flagged as poor quality0
Sequence length75
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACACTGATATATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAA2430010.2541936310865774TruSeq Adapter, Index 25 (97% over 44bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGTATG730150.058.8894945
CGTATGC745600.057.43782446
TATGCCG744950.057.316648
CTCGTAT749850.056.60146344
ACACGTC785750.056.1239613
GATCGGA737250.055.8792571
TATCTCG763400.055.79549441
TCTCGTA777650.054.4630643
ACGTCTG844100.052.187415
CACGTCT849300.051.90442714
ATCTCGT821850.051.57140442
ATCGGAA807800.051.0106662
CACACGT877100.050.40826412
GCCGTCT863550.049.28901351
CGTCTGA907200.048.5592516
ATGCCGT883900.048.2087549
GTATGCC892350.047.99200447
ATATATC890450.047.9538838
GATATAT905500.047.77326637
GCACACG942350.047.111911
Produced by FastQC (version 0.11.5)