FastQCFastQC Report
Wed 20 Apr 2022
EGAF00005431939

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005431939
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences95596809
Sequences flagged as poor quality0
Sequence length75
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATCA101000.017.28077750
GCCGTAT125050.014.56411548
CGCCGTA130000.013.71726147
GCGTCGT134900.013.28072712
CGTATCG84550.012.4058123
GATCGGA162200.012.3577292
GTATCGA89250.012.06189254
TCGCCGT153450.011.93576146
CGTCGTG170400.011.121313
AGGGGCG343800.010.60098110
TCGTGTA176800.010.55948515
CCCGTAT126800.010.5027721
TTACGCG27100.010.05530654
CGTGTAG197150.09.76585216
GTCGTGT194050.09.72954114
AGCGTCG186800.09.71986111
AGAGCGT189150.09.6328669
TGGTCGC187150.09.56563343
TCGGGGG493350.09.43852438
CCGGGAT211450.09.2841151
Produced by FastQC (version 0.11.5)