FastQCFastQC Report
Wed 20 Apr 2022
EGAF00005431940

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005431940
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences113043607
Sequences flagged as poor quality0
Sequence length50-75
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCGTAT184900.036.81045544
AGATCGG175050.030.0673831
TCGTATC258400.026.18188338
GATCGGA275050.025.6369671
TATCTCG270250.025.0769441
ATTCGTA271500.025.05012936
GTCACGA285650.024.0614229
CGTATCT287550.023.67176639
TCTCGTA293350.023.242343
AATTCGT294400.023.20231435
ACGAATT299050.023.0394132
ATCGGAA309450.022.8132782
CGAATTC302400.022.7255833
GAATTCG303400.022.7193334
CACGAAT303700.022.68578731
TTCGTAT307100.022.37805637
CGTCTGA315450.022.20922516
ACACGTC320050.021.95662913
AGTCACG325100.021.35128428
ATCTCGT323600.021.19929142
Produced by FastQC (version 0.11.5)