FastQCFastQC Report
Wed 20 Apr 2022
EGAF00005431941

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005431941
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences112985750
Sequences flagged as poor quality0
Sequence length50-75
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGTCGT134450.037.33526612
CGTCGTG146900.034.27237313
AGCGTCG151300.033.80475611
TCGTGTA162050.030.99742715
GTCGTGT172400.029.18661514
AGATCGG179400.028.9845311
CGTGTAG186000.027.3419916
GATCGGA190750.027.2477652
AGAGCGT211000.024.5935679
ATCGGAA218200.023.9580553
GAGCGTC235300.022.0282210
AAGAGCG240100.021.4792468
GATTAGT269700.018.88794737
TCGGAAG312700.016.9365184
CGGAAGA336150.015.7114045
TAGTGTA297950.014.50485240
AGATTAG370750.014.33246936
GTAGATC264500.014.20665544
GTAGGGA390900.013.51048319
GTGTAGG391550.013.47290517
Produced by FastQC (version 0.11.5)