FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005431944

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005431944
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences107913794
Sequences flagged as poor quality0
Sequence length75
%GC50

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGTATG237150.041.28351243
CGTATGC248550.039.31915344
CTCGTAT246050.039.2703742
TATGCCG263250.036.9733546
GATCGGA302150.033.4244421
ACGGCTA302100.032.40717332
ACACGTC321850.031.12757713
AGTCACG318100.030.7751728
ATCTCGT319100.029.7714640
TCGAACG254400.029.05968940
TTGAACG269850.028.7037357
ACGTCTG362550.027.77648515
TCTCGTA358050.026.41692441
GTCACGG386650.025.3820629
CGGCTAC386150.025.33519633
GTCGAAC295600.025.1607539
AACGGTA295100.025.02870843
CGGTAAC295850.025.02165645
ATCGGAA414750.024.5775432
ACGGTAA304700.024.2393144
Produced by FastQC (version 0.11.5)