FastQCFastQC Report
Wed 20 Apr 2022
EGAF00005431946

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005431946
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences67011376
Sequences flagged as poor quality0
Sequence length76
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTCCGCACATCTCGTA941620.14051644007429426TruSeq Adapter, Index 18 (97% over 40bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG227100.038.99519749
CTCGTAT229450.038.58982545
TCGTATG230900.038.4995546
ACGTCCG241200.037.74170733
CGTATGC240300.037.08072347
TCTCGTA253400.034.38999644
ACACGTC276850.032.939614
GATCGGA240000.032.877552
AGTCACG278500.032.8501429
CGCACAT269750.032.8396338
CGTCCGC282950.032.49413334
GTCACGT283900.032.23744230
AGATCGG243350.032.2282641
GTCCGCA284450.032.0515935
TCACGTC294250.031.31852531
ATCTCGT284800.030.87178843
ACGTCTG301500.030.36234716
ATGCCGT294850.029.86909950
TCCGCAC306700.029.85121536
ATCGGAA270100.029.5136363
Produced by FastQC (version 0.11.5)