FastQCFastQC Report
Wed 20 Apr 2022
EGAF00005431947

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005431947
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences67011376
Sequences flagged as poor quality0
Sequence length76
%GC49

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCC830350.12391179670747246Illumina Single End PCR Primer 1 (100% over 50bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATCA171700.046.2466550
GCCGTAT183900.043.36907648
CGCCGTA189550.041.984347
TCGCCGT199250.038.85473346
GATCGGA220000.038.6881872
GCGTCGT217800.038.37088812
CCGTATC211050.038.176949
CGTCGTG233350.035.95270513
TCGTGTA235050.034.72184415
GTCGTGT237250.034.37230314
AGAGCGT250500.034.0659759
AGATCGG250100.033.7880861
TGGTCGC231600.033.67351543
ATCGGAA253200.033.6471943
GTCGCCG241400.033.5595845
CGTGTAG247950.033.08570516
AGCGTCG255500.032.9702311
GAGCGTC276300.030.70869810
GGTCGCC265450.030.61172544
GTAGATC275500.030.5896132
Produced by FastQC (version 0.11.5)