FastQCFastQC Report
Wed 20 Apr 2022
EGAF00005431948

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005431948
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences152785913
Sequences flagged as poor quality0
Sequence length50-75
%GC48

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCGTAT617050.057.03767844
TCTCGTA770250.045.65238643
AATCTCG778400.045.28492741
GATCGGA763700.044.194021
ATCTCGT816750.043.07824342
ATCGGAA812450.041.6505132
ACACGTC832900.039.84829713
CGTCTGA833200.039.41354816
ACGTCTG862100.038.21218515
CACTCCG854800.037.14486731
ACTCCGG866800.036.6808632
CACGTCT922350.035.87195214
TCGGAAG966350.035.1669923
CCGGAGA942700.034.27686735
GCACACG981650.034.1391911
TCCGGAG938600.033.99125334
CACACGT987600.033.94240612
CTCCGGA948200.033.6012733
CGGAAGA1014500.033.3830954
CGGAGAA981150.033.18158336
Produced by FastQC (version 0.11.5)