FastQCFastQC Report
Wed 20 Apr 2022
EGAF00005431950

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005431950
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences90749436
Sequences flagged as poor quality0
Sequence length75
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGTATG230600.032.0742543
CTCGTAT235050.030.19119642
TATGCCG245950.030.01642846
CGTATGC250650.029.59115444
GATCGGA278150.027.9191991
AATCTCG272400.026.43047339
TCTCGTA282450.025.39374741
ACACGTC308700.025.01592613
ATCTCGT300000.023.97601540
ACGTCTG328400.023.61004315
ATCGGAA330050.023.3988512
CACGTCT371600.021.19906414
ATGCCGT365000.020.86713647
CACACGT377250.020.67019812
TCGGAAG396300.019.6786293
GCACACG406350.019.45272811
CCGTCTT395400.018.93139850
GCCGTCT400500.018.72449349
GACCAAT417650.018.30790335
GTATGCC410850.018.22947345
Produced by FastQC (version 0.11.5)