FastQCFastQC Report
Wed 20 Apr 2022
EGAF00005431951

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005431951
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences90749436
Sequences flagged as poor quality0
Sequence length75
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATCA171050.028.5632549
GCCGTAT188700.026.05471447
GCGTCGT218350.023.70402311
GATCGGA251500.023.1935521
AGCGTCG238950.023.06045310
CGTCGTG250600.020.5616312
AGAGCGT274250.020.399888
CGCCGTA246250.020.06372546
CCGTATC251350.019.7400248
TCGCCGT238200.019.4408945
ATCGGAA302000.019.317342
GTCGCCG270100.018.05046344
TGGTCGC254850.017.96733942
GTAGATC300250.017.91899931
GAGCGTC319700.017.6179819
AAGAGCG341150.017.0164287
GTATCAT299100.016.68043150
GTCGTGT311000.016.34019913
TCGGAAG363700.016.2895833
ATCTCGG361000.016.08085435
Produced by FastQC (version 0.11.5)