FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005431954

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005431954
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences64459010
Sequences flagged as poor quality0
Sequence length76
%GC48

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGTATG157050.026.2958946
CTCGTAT154800.025.68314445
TATGCCG171350.023.83956549
CGTATGC177200.023.4044547
TCTCGTA172400.022.71605944
CGTCCCG190300.022.6760736
ACCCGTC200950.021.73688733
AGATCGG171650.021.5285191
ACACGTC207850.021.24673314
GATCGGA174400.021.1569752
CCGATCT197100.020.31497240
GTCCCGA213200.020.17703637
ACGTCTG224750.019.8513316
ATCTCGT197100.019.8358543
CCCGATC206700.019.62544339
TCCCGAT218150.019.02841638
CACCCGT242950.018.13763432
ATCGGAA204750.018.1250613
CACGTCT252100.017.72544915
CACACGT260200.017.1603813
Produced by FastQC (version 0.11.5)