FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005431955

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005431955
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences64459010
Sequences flagged as poor quality0
Sequence length76
%GC48

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATCA110050.035.01173450
GCCGTAT117550.032.54000548
CGCCGTA131150.029.45877347
GCGTCGT140400.028.7614512
GATCGGA147400.027.9638672
CCGTATC142300.027.49841349
TCGCCGT143500.026.21847546
TGGTCGC151800.025.24863243
AGAGCGT168050.024.6810329
GTCGCCG161750.024.20942145
ATCGGAA174950.023.8426323
CGTCGTG168850.023.83459313
AGCGTCG171700.023.80478911
AGATCGG181350.022.5903111
GTAGATC186750.021.98342732
TCGTGTA186100.021.5304315
GTCGTGT196650.020.48276714
GAGCGTC205450.020.12856910
TCTCGGT205150.019.6225537
CGTGTAG207550.019.51760516
Produced by FastQC (version 0.11.5)