FastQCFastQC Report
Wed 20 Apr 2022
EGAF00005431968

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005431968
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences121441529
Sequences flagged as poor quality0
Sequence length75
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGTATG250950.026.11868143
CGTATGC262350.024.98326544
TATGCCG281150.023.39754546
CTCGTAT278550.023.09729442
GATCGGA316600.021.2754731
TCTCGTA310400.020.81582341
ACACGTC342400.019.96932213
ATCGGAA362500.018.5473292
ATCTCGT351050.018.4954740
ACGTCTG401400.017.08555415
CACGTCT418300.016.50259814
ATGCCGT431150.015.96096547
CACACGT443250.015.705667512
GTATGCC441300.015.38246545
CGTATCG97600.015.056653
GCCGTCT463350.014.49588349
TCGGAAG475650.014.483023
CCGTCTT474900.014.30296650
AGATCGG281600.014.2757071
GCACACG494650.014.14997611
Produced by FastQC (version 0.11.5)