FastQCFastQC Report
Wed 20 Apr 2022
EGAF00005431969

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005431969
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences121441529
Sequences flagged as poor quality0
Sequence length75
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATCA181750.021.80011250
GCCGTAT193900.020.5523848
CGCCGTA221650.017.74554447
GCGTCGT225900.017.69883712
TCGCCGT244700.016.2281746
TGGTCGC257450.015.57155443
GATCGGA266550.015.2701482
CCGTATC270700.015.07701949
CGTCGTG272000.015.01635613
AGCGTCG271550.014.76145411
AGATCGG277800.014.3621341
CGACCGT73700.014.1672281
CGTATCG95350.013.7853163
GTATCGA109700.012.9249974
AGAGCGT320200.012.791069
ATCGGAA319100.012.6602583
TCGTGTA327550.012.49457415
GTCGCCG338100.012.33651445
GTCGTGT347350.011.880548514
CCCGTAT152000.011.7823421
Produced by FastQC (version 0.11.5)