FastQCFastQC Report
Wed 20 Apr 2022
EGAF00005431970

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005431970
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences59751817
Sequences flagged as poor quality0
Sequence length100
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCGTAT119500.011.17661542-43
TATGCCG123950.010.83253646-47
CGTATGC129450.010.7157744-45
CGTATCG57400.010.7066693
CCGGGAT170850.09.9288651
AGATCGG486850.09.71820794
GATCGGA410400.09.62431794
CGCGTAT13700.09.603841
GTATCGA67100.09.578364
ACGGCTA140950.09.53895932-33
CCCGTAT86800.09.3655611
CGACCGT43150.08.9297751
CGGGTAT58250.08.8736891
CCGGCAT145050.08.8440741
CGGGGAT172350.08.833671
CGCGAAT32450.08.8332921
AGTCACG153950.08.82477228-29
CTCGGTT114250.08.5548931
CGCGGAT67650.08.5436751
ATCGGAA400150.08.48257594
Produced by FastQC (version 0.11.5)