FastQCFastQC Report
Wed 20 Apr 2022
EGAF00005431974

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005431974
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences124812316
Sequences flagged as poor quality0
Sequence length75
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATCA186600.017.5679750
GCCGTAT190100.017.27834547
CGACCGT84200.013.6338041
CGCCGTA251050.013.15221947
CGTATCG95200.012.9734953
GCGTCGT265550.012.8240311
CCGTATC260250.012.78965349
TCGCCGT262500.012.48584446
AGCGTCG295250.012.43090810
TGGTCGC267850.012.32641243
GTATCGA107700.012.1079534
GATCGGA314400.012.0756611
GTCGCCG292450.011.65500245
GTAGATC343900.011.24843731
CCCGTAT148450.011.226851
CGTCGTG318850.010.81045513
AGAGCGT354050.010.7111228
AGATCGG308900.010.6675841
ATCGGAA358550.010.630482
TCTATCG99250.010.15001162
Produced by FastQC (version 0.11.5)