FastQCFastQC Report
Wed 20 Apr 2022
EGAF00005431981

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005431981
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences99430395
Sequences flagged as poor quality0
Sequence length100
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACAGTTCCGTATCTCGTA2464060.24781758133415843TruSeq Adapter, Index 14 (97% over 44bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCGTAT476350.033.29615844-45
CGTATGC508100.031.30011446-47
TATGCCG512750.030.92847348-49
CCGTATC545450.029.03430238-39
TCGTATG471200.027.3113146-47
TTCCGTA584600.027.11236836-37
AGTTCCG593950.026.83950634-35
ATCTCGT604500.026.37712142-43
ATCGGAA974100.026.1690183
CGTATCT489600.026.12877540-41
TATCTCG496900.025.95875542-43
GATCGGA987750.025.8038272
GTTCCGT507450.025.19244836-37
TCGGAAG1075950.023.9273074
TCCGTAT534200.023.91479538-39
TCTCGTA549850.023.55697644-45
AGATCGG1099300.023.2221181
CGGAAGA1198400.021.6704675
GTATCTC755600.021.30259740-41
CACGTCT759350.021.30089414-15
Produced by FastQC (version 0.11.5)