FastQCFastQC Report
Wed 20 Apr 2022
EGAF00005431984

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005431984
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences94370651
Sequences flagged as poor quality0
Sequence length100
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATCG148500.021.2910613
GTATCGA159100.020.5211774
CCCGTAT189550.018.3186421
CCGTATC223800.015.5744082
CTCGTAT191750.015.23781742-43
CGACCGT71950.014.2363381
CGTATGC211600.014.051990544-45
TATGCCG224400.013.3242146-47
TATCGAA265700.013.1354885
ATCGAAG325250.012.2990416
ATCTCGT266000.011.51216840-41
CGCGGGT156400.011.476231
CCGGGAT224550.010.79715251
GACGCTA109400.010.4625054
CGGGGAT243850.010.4243051
ACGCTAC121050.09.9593995
TCGAAGG411400.09.6666167
CGCGTAT18750.09.5225871
CGGCAAT128200.09.3459781
CGGGGTT311200.09.2704621
Produced by FastQC (version 0.11.5)