FastQCFastQC Report
Wed 20 Apr 2022
EGAF00005431989

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005431989
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences80679033
Sequences flagged as poor quality0
Sequence length76
%GC48

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACAGTCAACAATCTCGTAT886350.10986125726122672TruSeq Adapter, Index 13 (97% over 40bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGATCGG242200.024.6584421
TCGTATG393500.024.5474145
GATCGGA309750.024.5167771
CGTATGC406900.023.58441446
CTCGTAT405450.023.53035744
TATGCCG412100.023.06060648
ACACGTC459150.022.25407413
TCTCGTA432250.022.08170943
AATCTCG445850.021.65897641
ATCGGAA356800.021.202422
ACGTCTG485200.021.1238815
ATCTCGT457000.020.85524642
CACGTCT505500.020.19267714
CACACGT512100.020.13089212
GCACACG534000.019.42325411
CGGAAGA516700.019.3245354
ATGCCGT493300.018.9243949
TCGGAAG421550.018.4043083
CCGTCTT518500.018.08595552
GTATGCC541100.018.01316647
Produced by FastQC (version 0.11.5)