FastQCFastQC Report
Wed 20 Apr 2022
EGAF00005431990

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005431990
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences80679033
Sequences flagged as poor quality0
Sequence length76
%GC48

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATCA158650.038.9261650
GCCGTAT163300.037.77473448
GCGTCGT181700.035.35341312
GATCGGA205550.032.1785242
CGCCGTA196100.031.56342747
AGCGTCG214200.030.35026411
CCGTATC208700.030.06418849
TGGTCGC202800.030.04421243
TCGCCGT215450.028.03270746
CGTCGTG241200.027.099713
AGAGCGT242800.026.8521699
GTCGCCG239050.026.59378245
GTAGATC251900.025.92158532
ATCGGAA256650.025.9126823
AGATCGG251750.025.8595161
TCGTGTA275200.024.07148415
TCTCGGT268900.024.02635637
GTATCAT276100.022.83651451
CGTGTAG290000.022.8012116
GTCGTGT293550.022.56503914
Produced by FastQC (version 0.11.5)