FastQCFastQC Report
Wed 20 Apr 2022
EGAF00005431991

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005431991
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences84220901
Sequences flagged as poor quality0
Sequence length76
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGTATG112900.017.11156544
CTCGTAT118000.016.6108643
TATGCCG119100.016.33833947
CGTATGC124950.015.51745245
TCTCGTA161800.012.48199442
AATCTCG168600.011.74987940
GATCGGA149350.011.5091742
CGCGAAT27400.010.8671191
ACACGTC188950.010.79696214
ATCTCGT197750.010.23024241
CCGGAAT133850.010.1806921
AGATCGG167400.010.1492131
ACGTCTG212650.09.75811416
CGGAAAT133550.09.5478061
ATCGGAA180800.09.4886013
CGCGGAT66850.09.3798891
ATGCCGT210550.09.35957948
CTCGGAT183600.09.3109331
CCCGTAT70900.09.2887581
CTCGAAT136650.09.2799371
Produced by FastQC (version 0.11.5)